منابع مشابه
dna-directed methylation analysis methodology
epigenetics alterations, especially dna methylation, play a critical role in control of gene expressions. abnormal patterns of methylation are observed in early-stages of many cancers. therefore, methylation analysis is useful in primary detection of tumors. advances knowledge about the functional role of aberrant epigenetic modifications as potential biomarkers for cancer, have attracted consi...
متن کاملDNA Methylation Analysis
Epigenetic mechanisms play important roles during normal development1, aging2, and a variety of disease conditions. Numerous studies have implicated aberrant methylation in the etiology of common human disease, including cancer, MS, diabetes, and schizophrenia3,4. Hypermethylation of CpG islands located in the promoter regions of tumor suppressor genes is firmly established as the most frequent...
متن کاملMethylation Analysis Using Methylation‐Sensitive HRM and DNA Sequencing
DNA methylation is a key epigenetic mechanism that regulates gene expression and chromatin structure. This application note describes a workflow for analysis of DNA methylation in specific loci by methylationsensitive high-resolution melting (MS-HRM) followed by DNA sequencing of the reaction products (Figure 1). The workflow relies on Applied Biosystems® real-time PCR and capillary electrophor...
متن کاملSerial pyrosequencing for quantitative DNA methylation analysis.
BioTechniques 721 In mammals, DNA methylation is a genetically programmed DNA modification and occurs almost exclusively at the 5-position of the pyrimidine ring of cytosines. Cytosine methylation is essential for mammalian embryogenesis and development, implicated in X inactivation, and influences gene expression of the corresponding genes, giving rise to distinct expressions patterns in diffe...
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ژورنال
عنوان ژورنال: Swiss Medical Weekly
سال: 2013
ISSN: 1424-7860,1424-3997
DOI: 10.4414/smw.2013.13799